The scheme came into effect on 1 October after MSPs voted to back the proposals.
The Coronavirus vaccine certification scheme will become subject to enforcement from today meaning higher risk venues and events affected must verify customers are fully vaccinated, or otherwise exempt, before entry.
The grace period from the October 1st launch date, allowing venues to test and trial the scheme without facing enforcement action, has now expired.
People attending a range of late night venues and larger indoor and outdoor live events, such as music festivals or large sporting events, will be required to show staff proof they are fully vaccinated or are otherwise exempt. This can be done via the NHS Scotland Covid Status App on their mobile device or using the paper certificate.
Health Secretary Humza Yousaf said:
"The grace period allowed venues and businesses affected more than two weeks to test the scheme in practice and make suitable arrangements. It has also provided the Government with helpful feedback from the sectors affected and we continue to liaise with them going forward.
"This is a very limited scheme and we hope this will allow businesses to remain open and prevent any further restrictions as we head into autumn and winter. This virus has not gone away and vaccine certification will have a role to play in keeping transmission under control as part of a wider package of measures. It adds a further layer of protection in certain higher risk settings.
"I also want to ensure that as many people get vaccinated as possible and particularly to increase uptake in the younger age cohort, so anything to incentivise that is helpful."