Network Rail is set to start the first phase of work on the reinstatement of passenger services to Levenmouth.
Engineers will be carrying out vegetation clearance and site survey and geological investigations to inform the development of the project - which will see the reinstatement of double tracks and two new stations for the east of Fife.
As part of the Scottish Government’s rail decarbonisation agenda, the line will also be prepared for future electrification.
Graeme Stewart, of Network Rail’s Levenmouth project team, said: "Although still at a very early stage, it is fantastic to see work happening literally preparing the ground and to inform the design of the line.
"We have been working on developing a range of options which will define what the project looks like and how it is delivered and, as part of this, we have been meeting with and listening to local groups and organisations in the area.
"The development and delivery of the project will be in discreet phases with the first visible work; removal of vegetation to enable site and geological investigation SI/GI the start of a process which will culminate on the community once again having access to the mainline rail network.
"As well as the promise of better connectivity this scale of investment to improve our transport infrastructure will help to deliver benefits to the economy.
"It will act as an enabler for growth, provide better access to employment and education opportunities and expanded social and leisure options for people all across the area."
A video showing part of the area to be reinstated - courtesy of Network Rail - can be seen above.