A cyclist, who was discovered with serious head injuries on the A91 on Monday evening, has died.
He has been identified as 43-year-old Scott Walker from St Monans.
Emergency services rushed Mr Walker to Ninewell's Hospital in Dundee shortly after 7:45pm on July 8, but later passed on Tuesday evening.
Police are still working to establish the full circumstances surrounding his death, but believe it could have been a result of a hit and run incident.
DCI John Anderson from Fife CID said:
"Our inquiries at the scene have indicated that at some point a Vauxhall Astra, we believe to be silver or partly silver in colour, has been involved in a collision.
"This could either be a hatchback or estate motor car or an Astra van.
"However, we do not know at this time if this is linked to the injuries Scott sustained, or if it this is a separate incident."
"We know from our initial CCTV enquires that a number of vehicles were on the road travelling in both directions between St.Monans and Elie between 7.30 and 7.45 on Monday night.
"We are actively working to identify these vehicles and therefore ask any motorists or members of the public who were on or around the A917 on Monday evening to get in touch with us as soon as possible.
"We would also be very keen to speak to anyone who believes they may have any dash-cam footage of this area from Monday evening."
He added: "Finally, I would appeal directly to the driver of any vehicle involved in this incident to come forward so that they can provide an explanation to us about what happened".
If you have any info please call 101 and quote incident number 3910 of 8th July. Alternatively call Crimestoppers on 0800 555 111.